Rapid method of total RNA mini-preparation from eucaryotic cells

Rapid method of total RNA mini-preparation from eucaryotic cells.

There have been described a wide variety of methods for the preparation of total RNA from eucaryotic cells (1, 2). These methods are mainly designed for the isolation of RNA from relatively large amounts of cells. In a situation of screening a large number of cell clones, minimized cell cultivation effort and preparation time for total RNA isolation will be of great advantage. We developed a me...

A rapid and efficient method for isolation of total RNA from Euglena gracilis (Euglenoidea).

RNA isolation is essential to the study of gene expression at the molecular level. However, it is difficult to isolate RNA from organisms that contain large amounts of polysaccharides or other compounds that bind or coprecipitate with RNA, such as the unicellular protist Euglena gracilis. Currently, there is no commercial kit available that is specific for the isolation of high-quality RNA from...

Rapid mini-preparation of fungal DNA for PCR.

RNAsnap™: a rapid, quantitative and inexpensive, method for isolating total RNA from bacteria

RNAsnap™ is a simple and novel method that recovers all intracellular RNA quantitatively (>99%), faster (<15 min) and less expensively (∼3 cents/sample) than any of the currently available RNA isolation methods. In fact, none of the bacterial RNA isolation methods, including the commercial kits, are effective in recovering all species of intracellular RNAs (76-5700 nt) with equal efficiency, wh...

RNA isolation (Total RNA Extraction from High Polysaccharide Plants). Fatemeh Mehrpooyan*